Unii parametri biochimici și morfopatologici la ovinele infestate spontan cu parazitoze cu localizare hepatică (Fasciola hepatica, Dicrocoelium lanceolatum, Echinococcus granulosus Larvae)

  • Nicolae NAFORNIȚĂ Universitatea Agrară de Stat din Moldova

Abstract

Abstract. The study was performed to determine some biochemical parameters in the blood and the concen­tration of liver marker enzymes in the blood serum, as well as micro- and macroelement level in sheep with liver parasitoses. The correlation of these parameters with morphopathological changes in the liver was also studied. Samples of blood were harvested from sheep to be slaughtered and the liver examination was performed after the slaughter of these sheep. The post-mortem examinations aimed to establish the invasions with Fasciola hepatica, Dicrocoelium lanceolatum and the presence of hydatid cysts with the establishment of morphopathological changes. After post-mortem examination the sheep were divided into groups with various infestation situations: control group; F. hepatica; D. lanceolatum; E. granulosus larvae; F. hepatica and D. lanceolatum; F.hepatica, D. lan­ceolatum and E. granulosus larvae. According to the study, the levels of total protein, total bilirubin and globulin showed a more or less pronounced increase, while the levels of albumin, magnesium and phosphorus significantly decreased. The parameters of the marker enzymes AST, ALT, ALP, GGT showed an increase of their concentration and activity in the blood serum, this fact being more accentuated in the groups with polyparasitism compared to the monoparasitized ones. The severity of morphopathological lesions were closely correlated with the type of parasitism: mono or polyparasitism and with the parasite load identified in the body of the studied sheep.


Key words: Sheep; Liver; Monoparasitism; Polyparasitism; Blood serum; Biochemical parameters; Morpho­pathological changes.


Rezumat. Studiul s-a realizat pentru a determina unii parametri biochimici ai sângelui și concentrația unor enzime marker ale ficatului din serul sangvin, precum şi nivelul micro- și macroelementelor la ovinele infestate cu parazitoze hepatice. S-a urmărit de asemenea corelarea acestor parametri cu modificările morfopatologice la nivelul ficatului. Probele de sânge s-au recoltat de la ovinele care urmau să fie sacrificate, iar examinarea ficatului s-a efectuat după sacrificarea acestor ovine. Examenele post-mortem au avut ca scop stabilirea invaziilor cu Fasciola hepatica, Dicrocoelium lanceolatum și a chisturilor hidatice, cu aprecierea modificărilor morfopatologice intervenite. Ovinele au fost divizate pe grupe, cu diverse situații de infestare: grupa control; F. hepatica; D. lanceolatum; E. granulosus larvae; F. hepatica și D. lanceolatum; F. hepatica, D. lanceolatum și E. granulosus larvae. Potrivit studiului, nive­lurile de proteină totală, bilurubină totală, globulină au prezentat o creștere mai mult sau mai puțin pronunțată, în timp ce nivelurile de albumină, magneziu și fosfor au scăzut semnificativ. Parametrii enzimelor marker AST, ALT, ALP, GGT au demonstrat o creștere a concentrației și activității lor în serul sangvin, fapt mai accentuat la grupele cu poliparazitism comparativ cu cele monoparazitate. Severitatea leziunilor morfopatologice a fost în strânsă corelație cu tipul de parazitism, mono- sau poliparasitism, și cu sarcina parazitară identificată în organismul ovinelor studiate.


Cuvinte-cheie: Ovine; Ficat; Monoparazitism; Poliparazitism; Ser sangvin; Parametri biochimici; Modificări morfopatologice.

References

1. ALTINTAŞ, N. (2003). Past to present: echinococcosis in Turkey. In: Acta Tropica, no. 85(2), pp.105-112. DOI: 10.1016/s0001-706x(02)00213-9.
2. ANDERSON, P.H., BERRETT, S., BRUSH, P.J., HEBERT, C.N., PARFITT, J.W., PATTERSON, D.S. (1977). Biochemical indicators of liver injury in calves with experimental fasciolasis. Veterinary Record, no. 100(3), pp. 43-45. DOI: 10.1136/vr.100.3.43.
3. BALKAYA, İ .et. al. (2009). Dicrocoelium dendriticum ile Enfekte Koyun Karaciğerleri Üzerinde Parazi¬tolojik ve Patolojik İncelemeler. In: Vet. Bil. Derg., vol. 4, pp. 169-175.Available: https://dergipark.org.tr/tr/ download/article-file/33811.
4. BALKAYA, İ., ŞİMŞEK, S. (2010). Erzurum’da Kesilen Sığırlarda Hidatidosis ve Fasciolosis’in Yaygınlığı ve Ekonomik Önemi. In: Kafkas Univ Vet Fak Derg., vol. 16(5), pp. 793-797, DOI:10.9775/kvfd.2010.1597
5. BENZER, F., TEMIZER OZAN, S. (2003). Fasciola hepatica ile enfekte koyunlarda lipit peroksidasyonu, an-tioksidan enzimler ve nitrik oksit düzeyleri. In: Turkish Journal of Veterinary and Animal Sciences, vol. 27(3), pp. 657-661. ISSN 657-661.
6. BUZA, Victoria, CHIHAI, Oleg, TĂLĂMBUŢA, Nina, NAFORNIŢĂ, Nicolae, VLASIUC, Ion. (2018). As¬pecte epidemiologice ale hidatidozei bovine în zona centru a Republicii Moldova. In: Materialele Simpozionu¬lui Internaţional „Ecologia funcţională a animalelor”, consacrat aniversării a 70 de ani de la naşterea academi¬cianului Ion Toderaş, 21 septembrie 2018. Chişinău, pp. 169-175. ISBN 978-9975-3159-7-5.
7. CAMARA, L., PFISTER, J., AESCHLIMANN, A. (1996). Analyse histopathologique de foie de bovin infeste´ par Dicrocoelium dendriticum. In: Veterinary Research, vol. 27, pp. 87–92. Available: https://hal.archives-ouvertes.fr/hal-00902402/document
8. CAMPO, R., MANGA-GONZALEZ, M.Y., GONZALEZ LANZA, C. (2000) Relationship between egg out¬put and parasitic burden in lambs experimentally infected with different doses of Dicrocoelium dendriticum. In: Veterinary Parasitology, vol. 87, pp. 139–149. DOI: 10.1016/s0304-4017(99)00165-x.
9. CERCEL, I., TOMŞA, M., NAFORNIŢA, N. (2018). Dicrocelioza la ovine, dinamica sezonieră de invadare a gazdei complementare cu metacercari. In: Lucrări ştiinţifice, Univ. Agrară de Stat din Moldova. Chişinău, vol. 49: Medicina Veterinară: Materialele Simpozionului Ştiinţific Internaţional „Probleme actuale şi tendinţe ale sectorului de creştere a animalelor şi medicinei veterinare”, dedicat aniversării a 85 de ani de la fondarea UASM, pp. 28-30. ISBN 978-9975-64-298-9.
10. DESCO, M. et. al. (1989). Blood biochemistry values of sheep (Ovis aries ligeriensis). In: Comparative Bio¬chemistry and Physiology, vol. 94(4), pp. 717-719. DOI: 10.1016/0300-9629(89)90622-1.
11. ERHAN, D. (2021). Tratat de parazitoze asociate ale animalelor domestice. Chişinău: Tipografia centrală. 1040 p. ISBN 978-9975-157-13-1.
12. EROL, Ayaz et. al. (2006). Endoparazitli Koyunlarda Bazı Biyokimyasal Parametreler. In: Türkiye Parazitoloji Dergisi, vol. 30(4), pp. 57-61.
13. JITHENDRAN, K.P., BHAT, T.K. (1996) Prevalence of dicrocoeliosis in sheep and goats in Himachal Pradesh, India. In: Veterinary Parasitology, vol. 61(3-4), pp. 265–271. DOI: 10.1016/0304-4017(95)00834-9.
14. HUNTLEY, J.F. et. al. (1984) The isolation and characterization of globule leucocytes: their derivation from mucosal mast cells in parasitized sheep. In: Parasite Immunology, vol. 6(4), pp. 371–390. DOI: 10.1111/j.1365- 3024.1984.tb00809.X.
15. İRAK, Kıvanç et. al. (2019). Investigation of Some Biochemical Parameters in Sheep Naturally Infected with Cystic Echinococcosis. In: KSU Journal Of Agriculture and Nature, vol. 22(6), pp. 948-952. DOI:10.18016/ ksutarimdoga.vi.529603.
16. KATAYAMA, F. (1996) Alterations of the mucin components in bile duct epithelium associated with prolif¬erating cholangitis. An experimental study (in Japanese). In: Hokkaido Igaku Zasshi, no. 71(5), pp. 585–598.
17. LEGESSE, D., GEMEDA, F. (2015) Comparison of Two Parasitological Tests and Post Mortem Examination to Detect Prevalence of Bovine Fasciolosis at Ambo Town Municipal Abattoir, West Shewa Zone, Ethiopia. In: Open Access Library Journal, no. 2, pp. 1-5. DOI: 10.4236/oalib.1101719.
18. MARTINEZ-MASENO, A.M., LUGUE, V.J., MORENO, T., REDONDO, E.S.H., MULAS, J., PERES, J. (1999). Liver pathology and immune response in experimental Fasciola hepatica infections of goats. In: Vet¬erinary Parasitology, vol. 82(1), pp. 19-33. DOI: 10.1016/s0304-4017(98)00262-3.
19. MALEK, E.A. (1980) Snail-transmitted parasitic diseases, vol. 2. CRC Press, 342 p. ISBN 9781315897554.
20. MANGA-GONZALEZ, M.Y., GONZALEZ-LANZA, C., CABANAS, E., CAMPO, R. (2001) Contributions to and review of dicrocoeliosis, with special reference to the intermediate hosts of Dicrocoelium dendriticum. In: Parasitology, vol. 123(7), pp. 91–114. DOI: 10.1017/s0031182001008204.
21. MANGA-GONZALEZ, M.Y., GONZALEZ-LANZA, C. (2005). Field and experimental studies on Dicrocoe¬lium dendriticum and dicrocoeliasis in northern Spain. In: Journal of Helminthology, vol. 79 (4), pp. 291–302, DOI: 10.1079/JOH2005323.
22. MEHLHORN, H., ed. (2001). Encyclopedic Reference of Parasitology: Biology, Structure, Function, 2nd ed. Berlin: Springer. 928 p. ISBN 978-3-540-29835-9.
23. MERT, N., KOZAT, S., EKIN, S., GÜNDÜZ, H., DENIZHAN, V. (2003). Doğal kronik Fasciolasisli koyun¬larda serum sialik asit ve serum lipid-bağlı sialik asit düzeyleri. In: Ulusal Parazitoloji Kongres.

24. OTRANTO, D., TRAVERSA, D. (2002). A review of dicrocoeliosis of ruminants including recent advances in the diagnosis and treatment. In: Veterinary Parasitology, vol. 107(4), pp. 317-335. DOI: 10.1016/s0304- 4017(02)00121-8.
25. OTRANTO, D., TRAVERSA, D. (2003). Dicrocoeliosis of ruminants: a little known fluke disease. In: Trends in Parasitology, no. 19(1), pp.12–15. DOI: 10.1016/s1471-4922(02)00009-0.
26. PERRY, B.D., RANDOLPH ,T.F. (1999). Improving the assesment of the economic impact of parasitic dis¬eases and of their control in production animals. In: Veterinary Parasitology, vol. 84 (3-4), pp. 145-168. DOI: 10.1016/s0304-4017(99)00040-0.
27. RANUCCI, S., GROL-RANUCCI, H. (1978). Ricerche emato-chimiche in ovini con lesioni parassitaire del fegato. In: Clinica Veterinaria, vol. 101, pp. 324–333.
28. RANUCCI, S., MUGHETTI, L., AMBROSI, M., GRELLONI, V. (1981). Rilievi ematochimici, anatomo-istopatologici e parassitologici in ovini infetti da dicroceliosi. In: Rivista di Zootecnia e Veterinaria (Italy), vol. 9(3), pp. 173-175.
29. SANCHEZ-CAMPOS, S. et. al. (1999). Oxidative stress and changes in liver antioxidant enzymes induced by experimental dicroceliosis in hamsters. In: Parasitology Research, vol. 85(6), pp. 468–474. DOI: 10.1007/ s00436005057938.
30. SANCHEZ-CAMPOS, S. et. al. (2000). Morphologic and biochemical changes caused by experimentally in¬duced dicroceliosis in hamsters (Mesocricetus auratus). In: Comparative Medicine, vol. 50(2), pp.147–152. ISBN 1532-0820. https://www.researchgate.net/publication/12461667_Morphologic_and_biochemical_ changes_caused_by_experimentally_induced_dicroceliosis_in_hamsters_Mesocricetus_auratus.
31. SANCHEZ-ANDRADE, R., PAZSILVA, A., SUAREZ, J.L., ARIAS, M., LOPEZ, C., MORRONDO, P., SCA¬LA, A. (2003). Serum antibodies to Dicrocelium dendriticum in sheep from Sardinia (Italy). In: Preventive Veterinary Medicine, vol. 57(1-2), pp. 1-5. DOI: 10.1016/s0167-5877(02)00215-5.
32. SANCHEZ-COMPOS, S., TUNAN, M.J., GONZALES, P., MARIN, J.J.G., GONZALES-GALLEGO, J. (1998). Enhanced bile formation induced by experimental dicrocoeliosis in the hamster. In: Life Science, vol. 63, pp. 1963-1974. Available: https://doi.org/10.1016/S0024-3205(98)00474-3.
33. SCHANTZ, P.M. et. al. (1995). Epidemiology and control of hydatit diseases. In: R.C.A. Thomson, A.J.L. Limberg, eds. Echinococcus and Hydatid Disease, UK, Bridless ltd, Guilford, pp. 231-331.
34. SINCLAIR, K.B. (1967). Pathogenesis of Fasciola and other liver-flukes. In: Helminthological Abstracts, vol. 36(2), pp. 115-34.
35. THEODORIDIS, Y., DUNCAN, J.L., MACLEAN, J.M., HIMONAS, C.A. (1991). Pathophysiological studies on Dicrocoelium dendriticum infection in sheep. In: Veterinary Parasitology, vol. 39(1-2), pp. 61–66. https:// doi.org/10.1016/0304-4017(91)90062-Z.
36. TOMŞA, M., NAFORNIŢA, N. (2011). Indicii biochimici ai organelor și țesuturilor de origine bovină în cazul afecțiunilor parazitare. In: Culegeri de lucrări ale Simpozionului științific cu participare internaţ., IŞPBZMV, Maximovca, 2011, pp. 262-264. ISBN 978-9975-56-004-7.
37. YAMASHIRO, M., KOUDA, W., KONO, N., TSUNEYAMA, K., MATSUI, O., NAKANUMA, Y. (1998). Distribution of intrahepatic mast cells in various hepatobiliary disorders. In: An immunohistochemical study. Virchows Arch, vol. 433(5), pp. 471–479. DOI: 10.1007/s004280050276.
38. АБАЛИХИН, Б.Г. (1982). Динамика активности ентерокиназов и щелочной фосфотазы кишечника романовских овец в норме и при экспериментальном дикроцелиозе. В: Сборник научных трудов МВА К.И. Скрябина, vol. 124, pp. 46-48.
39. ДЕМЕНТЬЕВ, И.С. (1970). Некоторые вопросы биологии и особенности эпизоотологии дикроцелиоза овец на юго-востоке Казахстана: Автореферат дис. канд. вет. наук. Оренбург, 20 с.
40. КУБЛИЦКЕНЕ, О. (1976). Патоморфология, лечение и восстановительные процессы при экспериментальном фасциолезе. Вильнюс: Мокслас, с. 160.
Published
2021-08-30
How to Cite
NAFORNIȚĂ, Nicolae. Unii parametri biochimici și morfopatologici la ovinele infestate spontan cu parazitoze cu localizare hepatică (Fasciola hepatica, Dicrocoelium lanceolatum, Echinococcus granulosus Larvae). Stiinta agricola, [S.l.], n. 1, p. 137-147, aug. 2021. ISSN 2587-3202. Available at: <https://sa.uasm.md/index.php?journal=sa&page=article&op=view&path%5B%5D=749>. Date accessed: 02 dec. 2021.
Section
Table of contents