Indicii microbiologici ai familiilor de albine înaintea iernatului

Microbiological indices of bee colonies before wintering

  • Veronica BUGNEAC Universitatea Agrară de Stat din Moldova


Bee colonies are exposed to a wide variety of actions of the agricultural ecosystem and to a multitude
of variations of the environment throughout the year. All these factors can affect the bees’ microbial balance. This study aimed to determine the microflora of bee families before the wintering period. The bees for the research were randomly collected at two apiaries of 17 and 24 bee families. Under laboratory conditions, insemination was performed on common, selective and differential growth media using material of the bees’ intestines. As a result of the investigations, an intensive growth of bacterial colonies was noticed on all microbial culture media. They were predominantly formed by different forms of bacteria such as streptococci, staphylococci, E. coli, Salmonella and microscopic fungi. Most colonies of microorganisms developed on Endo and Peptonate Agar media, being represented by the microbial colonies of E. coli and streptococci, respectively.
Key words: Honey bees; Intestinal microflora; Nutrient media; Microbial colonies.
Rezumat. Coloniile de albine pe tot parcursul anului sunt expuse la o varietate largă de acțiuni ale ecosistemelor agricole și la o multitudine de variații ale mediului, care le pot afecta echilibrul microbian. Studiul de faţă îşi propune de a stabili varietatea microflorei familiilor de albine înaintea perioadei de iernat. Albinele pentru cercetare au fost colectate în mod aleatoriu de la două stupine, cu 17 şi 24 de familii de albine. În condiții de laborator, din intestinele albinelor au fost efectuate însămânțări pe medii nutritive obișnuite, selective și diferențiale. În rezultatul investigațiilor s-a stabilit o creștere intensivă de colonii bacteriene pe toate mediile microbiene nutritive. Acestea au fost constituite preponderent din formele bacteriene precum streptococi, stafilococi, E. coli, salmonele și fungi microscopici. Cele mai multe colonii de microorganisme s-au dezvoltat pe mediile Endo și Agarul peptonat, fiind reprezentate de coloniile microbiene E. coli și, respectiv, streptococi.
Cuvinte-cheie: Albine melifere; Microfloră intestinală; Medii nutritive; Colonii microbiene. 


1. BERRY, J.A., HOOD, W.M., PIETRAVALLE, S., DELAPLANE, K.S. (2013). Field-level sublethal efects of approved bee hive chemicals on honey bees (Apis mellifera L). Available: PLoS ONE 8(10): e76536.
2. BURA, M., PĂTRUICĂ, S., BURA, A. (2013). Tehnologie apicolă. Timișoara: Solness. 380 p.
3. CARRILO-TRIPP, J., DOLEZAL, A.G., GOBLIRSCH, M.J. et al. (2016). In vivo and in vitro infection dynamics of honey bee viruses. In: Scientific Reports, vol. 6, pp. 1-12. DOI 10.1038/srep22265
4. EVENS, J., LOPEZ, D. (2004). Bacterial probiotics induce an immune response in the honey bee. (Hymenoptera: Apidae). In: Journal of Economic Entomology, vol. 97, pp. 752-756. ISSN 0022-0493.
5. GOULSON, D., NICHOLLS, E., BOTIAS, C., ROTHERAY, E.L. (2015). Bee declines driven by combined stress from parasites, pesticides, and lack of fowers. In: Science, vol. 347, issue 6229. DOI: 10.1126/science.1255957
6. MARGHITAS, L.A. ( 2005). Albinele şi produsele lor. Bucureşti: Ceres. 387 p.
7. NAZZI, F. et al. (2012). Synergistic parasite–pathogen interactions mediated by host immunity can drive the collapse of honeybee colonies. In: PLoS Pathog 8(6): e1002735.Available:
8. OLOFSSON, T., VASQUEZ, A. (2008). Detection and identification of a novel lactic and bacterial flora within the honey stomach of the honey bee Apis mellifera. In: Microbiology, vol. 57(4), pp. 356-363. ISSN 0343-8651.
9. OTTO, C.R., ROTH, C.L., CARLSON, B.L., SMART, M.D. (2008). Land-use change reduces habitat suitability for supporting managed honey bee colonies in the Northern Great Plains. In: Proceedings of the National Academy of Sciences of the USA, vol. 113(37), pp. 10430–10435.
10. TARPY, D.R., SEELEY, T.D. (2006). Lower disease infections in honey bee (Apis mellifera) colonies headed by polyandrous vs monandrous queens. In: Naturwissenschafen, vol. 93, pp. 195–199. ISSN 0028-1042.
How to Cite
BUGNEAC, Veronica. Indicii microbiologici ai familiilor de albine înaintea iernatului. Stiinta agricola, [S.l.], n. 2, p. 125-128, jan. 2018. ISSN 2587-3202. Available at: <>. Date accessed: 31 mar. 2020.
Table of contents