Screening-ul germoplasmei de floarea-soarelui la rugină

  • Maria DUCA Universitatea Academiei de Ştiinţe a Moldovei
  • Tatiana ŞESTACOVA Universitatea Academiei de Ştiinţe a Moldovei
  • Angela PORT Universitatea Academiei de Ştiinţe a Moldovei
  • Aliona CUCEREAVÎI „AMG – Agroselect” SRL, Republica Moldova
  • Ion GÎSCĂ „AMG – Agroselect” SRL, Republica Moldova
  • Olesea TABĂRĂ Universitatea Academiei de Ştiinţe a Moldovei

Abstract

A key feature of sunflower commercial hybrids is the resistance to various diseases and pests, which excludes the use of chemical treatments and reduces negative environmental impacts. Rust is a fungal disease caused by Puccinia helianthi leading to significant yield losses. The molecular screening of rust resistance genes offers the possibility of identifying parental forms and hybrids resistant to infection. In this study 42 sunflower genotypes (Rf paternal lines, maternal lines with cytoplasmic male sterility (CMS) and F1 commercial hybrids) were evaluated for the presence of the amplicon 950 pb associated with R1 gene, which confer resistance to the rust race 100. Molecular SCAR analysis (Sequence Characterized Amplified Regions) showed that the amplicon was not present at CMS lines indicating that these genotypes do not possess R1 gene. The fragment of 950 bp was found only in 13 genotypes: 10 paternal lines and three local commercial F1 hybrids (Nistru, Doina and Oscar). The data provide useful information to breeders for selecting parental combinations to create hybrids resistant to rust.


Key words: Helianthus annuus; Screening; Molecular marker; Rust; Resistance.


Rezumat: O caracteristică esenţială a hibrizilor comerciali de floarea-soarelui reprezintă rezistenţa la diferite boli şi dăunători, ceea ce exclude utilizarea tratamentelor chimice şi diminuează impactul negativ asupra mediului. Rugina reprezintă o maladie micotică provocată de Puccinia helianthi ce cauzează pierderi semnificative de recoltă. Screening-ul molecular al genelor de rezistenţă la rugină oferă posibilitatea identificării formelor parentale şi hibrizilor rezistenţi la infecţie. În acest studiu s-au evaluat 42 genotipuri de floarea-soarelui (linii paterne Rf, linii materne cu androsterilitate citoplasmatică (ASC) şi hibrizi F1 comerciali) pentru a se stabili prezenţa ampliconului de 950 pb asociat cu gena R1, ce conferă rezistenţă la rasa de rugină 100. Analiza SCAR (Sequence Characterized Amplified Regions) a demonstrat lipsa ampliconului de 950 pb la toate liniile materne, ceea ce indică faptul că aceste genotipuri nu posedă gena R1 . Fragmentul de 950 pb a fost identificat doar la 13 genotipuri de floarea-soarelui: 10 linii paterne şi 3 hibrizi comerciali autohtoni F1 (Nistru, Doina şi Oscar). Datele obţinute furnizează informaţii utile amelioratorilor privind selectarea combinaţiilor parentale în crearea hibrizilor rezistenţi la rugină.


Cuvinte cheie: Helianthus annuus; Screening; Marker molecular; Rezistenţă; Rugină.

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Published
2017-05-01
How to Cite
DUCA, Maria et al. Screening-ul germoplasmei de floarea-soarelui la rugină. Stiinta agricola, [S.l.], n. 2, p. 15-19, may 2017. ISSN 2587-3202. Available at: <https://sa.uasm.md/index.php?journal=sa&page=article&op=view&path%5B%5D=129>. Date accessed: 24 oct. 2020.
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