Screening-ul germoplasmei de floarea-soarelui la rugină

  • Maria DUCA Universitatea Academiei de Ştiinţe a Moldovei
  • Tatiana ŞESTACOVA Universitatea Academiei de Ştiinţe a Moldovei
  • Angela PORT Universitatea Academiei de Ştiinţe a Moldovei
  • Aliona CUCEREAVÎI „AMG – Agroselect” SRL, Republica Moldova
  • Ion GÎSCĂ „AMG – Agroselect” SRL, Republica Moldova
  • Olesea TABĂRĂ Universitatea Academiei de Ştiinţe a Moldovei

Abstract

A key feature of sunflower commercial hybrids is the resistance to various diseases and pests, which excludes the use of chemical treatments and reduces negative environmental impacts. Rust is a fungal disease caused by Puccinia helianthi leading to significant yield losses. The molecular screening of rust resistance genes offers the possibility of identifying parental forms and hybrids resistant to infection. In this study 42 sunflower genotypes (Rf paternal lines, maternal lines with cytoplasmic male sterility (CMS) and F1 commercial hybrids) were evaluated for the presence of the amplicon 950 pb associated with R1 gene, which confer resistance to the rust race 100. Molecular SCAR analysis (Sequence Characterized Amplified Regions) showed that the amplicon was not present at CMS lines indicating that these genotypes do not possess R1 gene. The fragment of 950 bp was found only in 13 genotypes: 10 paternal lines and three local commercial F1 hybrids (Nistru, Doina and Oscar). The data provide useful information to breeders for selecting parental combinations to create hybrids resistant to rust.


Key words: Helianthus annuus; Screening; Molecular marker; Rust; Resistance.


Rezumat: O caracteristică esenţială a hibrizilor comerciali de floarea-soarelui reprezintă rezistenţa la diferite boli şi dăunători, ceea ce exclude utilizarea tratamentelor chimice şi diminuează impactul negativ asupra mediului. Rugina reprezintă o maladie micotică provocată de Puccinia helianthi ce cauzează pierderi semnificative de recoltă. Screening-ul molecular al genelor de rezistenţă la rugină oferă posibilitatea identificării formelor parentale şi hibrizilor rezistenţi la infecţie. În acest studiu s-au evaluat 42 genotipuri de floarea-soarelui (linii paterne Rf, linii materne cu androsterilitate citoplasmatică (ASC) şi hibrizi F1 comerciali) pentru a se stabili prezenţa ampliconului de 950 pb asociat cu gena R1, ce conferă rezistenţă la rasa de rugină 100. Analiza SCAR (Sequence Characterized Amplified Regions) a demonstrat lipsa ampliconului de 950 pb la toate liniile materne, ceea ce indică faptul că aceste genotipuri nu posedă gena R1 . Fragmentul de 950 pb a fost identificat doar la 13 genotipuri de floarea-soarelui: 10 linii paterne şi 3 hibrizi comerciali autohtoni F1 (Nistru, Doina şi Oscar). Datele obţinute furnizează informaţii utile amelioratorilor privind selectarea combinaţiilor parentale în crearea hibrizilor rezistenţi la rugină.


Cuvinte cheie: Helianthus annuus; Screening; Marker molecular; Rezistenţă; Rugină.

References

1. BACHLAVA, E., RADWAN, O.E., ABRATTI, G.et al., 2011. Downy mildew (Pl8 and Pl14) and rust (RAdv) resistance genes reside in close proximity to tandemly duplicated clusters of non-TIR-like NBS-LRR-encoding genes on sunflower chromosomes 1 and 13. In: Theoretical and Applied Genetics, vol. 122, pp. 1211-1221.
2. BAILEY, D.L., 1923. Sunflower rust. University of Minnesota Agricultural Experiment Station, Technical Bulletin 16, 39 p. Disponibil: http://conservancy.umn.edu/bitstream/handle/11299/139550/1/TB16.pdf
3. FICK,G.N., 1983. Genetics breeding of sunflower. In: Journalof Oil and Fat Industies, vol. 60 (7), pp. 1252-1253.
4. GONG,L., GULYA, T.J., MARKELL, S.G., HULKE, B.S., QI, L.L., 2013a. Genetic mapping of rust resistance genes in confection sunflower line HA-R6 and oilseed line RHA 397. In: Theoretical and Applied Genetics, vol. 126, pp. 2039-2049.
5. GONG, L., HULKE, B.S., GULYA, T.J., MARKELL, S.G., QI, L.L., 2013b. Molecular tagging of a novel rust resistance gene R12 in sunflower (Helianthus annuus L.) In: Theoretical and Applied Genetics, vol. 126, pp. 93-99.
6. GULYA, T.J., MARKELL, S., 2009. Sunflower rust status -2008. Race frequency across the midwest and resistance among commercial hybrids, Fargo. Disponibil: http://www.sunflowernsa.com/uploads/resources/195/gulya_ruststatus_09.pdf
7. GULYA, T., VENETTE, R., VENETTE, J. R., LAMEY, H. A., 1990. Sunflower rust. North Dakota State University, NDSU Extension Service, PP-998. Disponibil: http://www.ext.nodak.edu/extpubs/plantsci/rowcrops/pp998.pdf
8. LAWSON, W.R., GOULTER, K.C., HENRY, R.J., KONG, G.A., KOCHMAN, J.K., 1998. Marker assisted selection for two rust resistance genes in sunflower. In: Molecular Breeding, nr. 4, pp. 227-234.
9. LAWSON, W.R., JAN, C.C., SHATTE, T., SMITH, L., KONG, G.A., KOCHMAN, J.K., 2011. DNA markers linked to the R2 rust resistance gene in sunflower (Helianthus annuus L.) facilitate anticipatory breeding for this disease variant. In: Molecular Breeding, nr. 28, pp. 569-576.
10. MORRIS, J.B., YANG, S.M., WILSON, L., 1983. Reaction of Helianthus species to Alternaria helianthi. In: Plant Disease, nr. 67, pp. 539-540.
11. PERESYPKIN, V.F., 1989. Sel‘skohozâjstvennaâ fitopatologiâ. Moskva: Agropromizdat. 480 s.
12. PUTT, E.D., SACKSTON, W.E., 1963. Studies onsunflower rust IV. Twogene, R1 and R2 for resistance in the host. In: Canadian Journal of Plant Science, vol. 43 (4), pp. 490-496.
13. QI, L.L., GULYA, T.J., HULKE, B.S., VICK, B.A., 2012a. Chromosome location, DNA markers and rust resistance of the sunflower gene R5. In: Molecular Breeding, nr. 30, pp. 745-756.
14. QI ,L.L., GULYA, T.J., SEILER, G.J., HULKE, B.S., VICK, B.A., 2011a. Identification of resistance to new virulent races of rust in sunflowers and validation of DNA markers in the gene pool. In: Phytopathology, vol. 101, pp. 241-249.
15. QI, L.L., HULKE, B.S., VICK, B.A., GULYA, T.J., 2011b. Molecular mapping of the rust resistance gene R4 to a large NBS-LRR cluster on linkage group 13 of sunflower. In: Theoretical and Applied Genetics, vol. 123, pp. 51-358.
16. QI, L.L., SEILER, G. J., VICK, B.A., GULYA, T. J., 2012b. Genetics and mapping of the R11 gene conferring resistance to recently emerged rust races, tightly linked to male fertility restoration, in sunflower (Helianthus annuus L.). In: Theoretical and Applied Genetics, vol. 125, pp. 921-932.
17. QURESH, Z., JAN, C.C., GULYA, T.J., 1993. Resistance of sunflower rust and its inheritance in wild sunflower species. In: Plant Breeding, vol. 119, pp. 297-306.
18. RASHID, K.Y., DESJARDINS, M., KAMINSKI, D.A., 2003. Diseases of sunflower in Manitoba in 2002. In: The Canadian Plant Disease Survey, vol. 83, pp. 133-134.
19. ŞESTACOVA, T., 2011. Molecular screening of R1 rust resistance gene among different sunflower genotypes cultivated in Republic of Moldova. In: Structura şi funcţionalitatea sistemelor biologice -diversitate şi universalitate: materialelec onf. şt., 17 noiem. 2011, pp. 57-60.
20. ŠKORIĆ, D., 1985. Sunflower breeding for resistance to Diaporthe/Phomopsishelianthi. In: Helia, nr. 8, pp. 21-23.
21. VRONSKIH, M.D., PЕТКОVIČ, I.P., 2007. Bolezni podsolnečnikai mery bor‘by snimi. Chişinău. 68 s. ISBN 978-9975-9521-8-7.
Published
2017-05-01
How to Cite
DUCA, Maria et al. Screening-ul germoplasmei de floarea-soarelui la rugină. Stiinta agricola, [S.l.], n. 2, p. 15-19, may 2017. ISSN 2587-3202. Available at: <https://sa.uasm.md/index.php?journal=sa&page=article&op=view&path%5B%5D=129>. Date accessed: 11 dec. 2024.
Citation Formats
Issue
No 2 (2014): Știința agricolă
Section
Table of contents
Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Most read articles by the same author(s)