Monitoringul coloniilor microbiene izolate de la carcasele de bovine, ovine şi porcine

  • Ruslan ANTOCI Universitatea Agrară de Stat din Moldova

Abstract

A study, conducted during 2013-2014, aimed at monitoring the number of microorganisms isolated from bovine, sheep and pig carcasses and grown on nutritive media: peptone agar, Endo, bismuth sulfite agar and Saburo. The samples were taken from the surface, as well as from the depth of carcasses (from livestock farms from different districts of the Republic of Moldova), during their marketing period. Microbial dominant species from carcasse surface were represented by E. coli and Streptococcus. The greatest number of microorganism colonies were found on the samples from sheep and pig carcasses, the contamination level being higher in the months of April and July. The number of colonies taken from the surface of carcasses, compared with those taken from deep muscle, was 2-3 times higher with a higher incidence on peptone agar and Endo media: up to 66 colonies (sheep), 38 colonies (pig) şi 31 colonies (cattle). The number of colonies identified on bismuth sulfite agar and Saburo media was more reduced.


Key words: Carcasses; Cattle; Sheep; Pigs; Bacteria; Nutritive media.


Rezumat. Rezultatele prezentate în lucrare se referă la monitorizarea numărului de microorganisme izolate de la carcasele de bovine, ovine şi porcine şi crescute pe medii nutritive: agarul peptonat, Endo, bismut sulfit agar şi Saburo. Probele au fost prelevate de pe suprafaţa şi din profunzimea carcaselor (provenite de la unităţi zootehnice din diferite raioane ale republicii), în perioada de comercializare a acestora (2013-2014). Cercetările au stabilit că dominanta microbiană de pe suprafaţa carcaselor constă în primul rând din E. coli şi streptococci. Cel mai mare număr de colonii de microorganisme a fost determinat în probele prelevate de la carcasele de ovine şi porcine, nivelul de contaminare fiind mai ridicat în lunile aprilie şi iulie. Numărul coloniilor de pe suprafaţa carcaselor, comparativ cu cele din profunzime, a fost de 2-3 ori mai mare, cu oincidenţă mai evidentă pe mediile agar peptonat şi Endo: pâna la 66 colonii (ovine), 38 colonii (porcine) şi 31 colonii (bovine). Numărul coloniilor a fost mai redus pe mediile bismut sulfit agar şi Saburo.


Cuvinte-cheie: Carcase; Bovine; Ovine; Porcine; Bacterii; Medii nutritive.

References

1. ABDALLA, M.A., SULIMAN, S.E., BAKHIET, A.O. (2010). Method for reducing contamination of indigenous cattle carcasses during slaughtering. In: Assiut Veterinary Medical Journal, vol. 56(127), pp. 86-93. ISSN 10125973.
2. BOLTON, D.J, IVORY, C., McDOWELL, D. (2013). A study of Salmonella in pigs from birth to carcass: serotypes, genotypes, antibiotic resistance and virulence profiles. In: International Journal of Food Microbiology, 160(3), pp. 298-303.
3. COOMAN, L, FLAHOU, B., HOUF, K., SMET, A., DUCATELLE, R., PASMANS, F., HAESEBROUCK, F. (2013). Survival of Helicobacter suis bacteria in retail pig meat. In: International Journal of Food Microbiology, vol. 166(1), pp. 164-167.
4. DAN, S. D., ROTARU, O., DALEA, I. (2006). The effect of lactic and acetic acid treatment on psychrotrophic germ growth from the surface of beef and pork. In: Bulletin UASVM Cluj-Napoca. Veterinary Medicine, vol. 63(12), pp. 245-250. ISSN 1454-2382.
5. DAN, S.D., ROTARU, O., FILIPOI, C. (2004). Comparative morphological researches of Sarcocystis spp. microcysts in sheep meat and pork. In: Bulletin UASVM Cluj-Napoca, vol. 61, pp. 295-296. ISSN 1454-2382.
6. DAN, S.D., ROTARU, O., RĂPUNTEAN, Gh., MIHAIU, M., ZEGREAN, G. (2003). The dynamic of psychrotrophic microflora in beef during slaughtering process. In: Bulletin UASVM Cluj-Napoca, vol. 60, pp. 6771. ISSN 1454-2382.
7. IVANA, S., BOGDAN, A.T., ŢOGOE, I., CÂMPEANU, Gh., IUDITH, Ipate, POPESCU, N. (2010). Microbiologia alimentelor. Vol. 3. Bucureşti: Editura Asclepius. 270 p. ISBN 978-973-88785-8-7, 978-606-92360-3-1.
8. MIHAIU, M., FAZEKAS, I., LAPUSAN, A., MIHAIU, R., DAN, S.D., TAULESCU, C., MIHAIU, L. (2010). The antimicrobial resistance of Escherichia coli isolated from meat and meat products. In: 10th International Symposium “Prospects for the 3rd Millennium Agriculture”, UASVM Cluj-Napoca, 28 September 2010, Romania.
9. TAHAMTAN, Y., HAYATI, M., NAMAVARI, M. (2010). Contamination of sheep carcasses with verocytotoxin producing Escherchia coli during slaughtering. In: Transboundary and Emerging Diseases, vol. 57(1-2), pp. 25-27.
Published
2017-05-01
How to Cite
ANTOCI, Ruslan. Monitoringul coloniilor microbiene izolate de la carcasele de bovine, ovine şi porcine. Stiinta agricola, [S.l.], n. 1, p. 112-118, may 2017. ISSN 2587-3202. Available at: <https://sa.uasm.md/index.php?journal=sa&page=article&op=view&path%5B%5D=120>. Date accessed: 13 may 2021.
Section
Table of contents